Comes with PDI insert and 50 self-adhesive biocompatible gaskets, which allow to use the assembly with regular 35 mm Petri dishes
Cells cultured in 35 mm Petri dishes are a popular research tool used in numerous applications, including patch clamping and intracellular ion probe imaging. However, true perfusion (continuous inflow and outflow) of solutions can be difficult to configure. Drug delivery without an outflow requires a spritzer-type microinjector, but ultimately results in contamination of the entire dish after only a few applications. This forces scientists to plate cells on cover slips for placement into specially designed perfusion chambers. However, such transfer is a time consuming process which introduces the potential for contamination plus additional expense
The PDI chamber was designed by scientists after several years of patch clamp research and calcium imaging combined with external perfusion of single cells cultured in Petri dishes. The chamber has separate openings for solution inflow and outflow that dump the fluctuations of the liquid level in the working compartment and prevents bubbles from entering the chamber. The laminar profile facilitates perfusion and provides faster solution exchange. An adjustable metal suction tube (included) controls the level of liquid in the dish. The chamber was designed for use with perfusion systems and included magnetic holder with miniature ball-joint can accommodate perfusion manifolds (optional). Can be used with glass bottom dishes for imaging. In fact, some glass bottom dishes are made from standard Petri dishes like Corning 35 mm, for example (Mattek dishes). Can be used with temperature controlled systems. The suction tubing requires connection to an outflow source CFPS-1U
See published sample recordings, and Sample publications.
Includes three magnetic tubing / electrode holders, and stainless suction tubing. A magnetic microscope adapter is required. Can be used with both magnetic and non-magnetic microscope adapters
If used with non-magnetic adapters, a IMA-MH set of miniature holders is required
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Specifications:
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Outside diameter:
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35 mm
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Height:
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3 mm
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Working volume:
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11 mm diameter, approx. 100 microl
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