Koehler et al developed the Visual Stimuli Box to evaluate mouse vision through looming visual stimuli. The setup includes a square enclosure with an opaque shelter, a ceiling-mounted computer monitor for projecting stimuli, and a camera for recording behavior (monitor and camera optional).

The looming stimulus is an expanding black circle on the monitor. Mice are placed in the enclosure to acclimate before being exposed to the stimulus, and their responses (flight or freeze) are recorded and analyzed with motion-tracking software. This apparatus is compatible for tracking and analysis using video tracking packages such as ConductVision, Noldus EthoVision, or ANY-Maze

This device is a simple, reliable tool for assessing vision-related behavioral responses, focusing on image-forming pathways in the central visual system

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