telefipho
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TeleFipho

Fiber photometry is a powerful technique to detect calcium signal from specific neuron in awake animals using calcium indicator protein represented by GCaMP

In freely moving condition, the long optic fiber attached to the head of the animal can interfere with experimental setup, therefore can be a limitation factor of your project

Our innovative new product, TeleFipho, includes all required components for fiber photometry - optic fiber, filter cube, light source, photo detector - and also wireless transmission circuit, in the very smal 3g body

TeleFipho definitely will not block free behavior of your animals, enabling novel experimental approach using fiber photometry

Features

  • World first commercial wireless fiber photometry
  • Small headstage / good for mice, rats, marmosets, etc.
  • Standard 2.5mm ferrule cannula
  • Rechargable by a dedicated charger
  • Adjustable excitation LED power
  • Adjustable signal offset
  • For GCaMP or GFP-like indicators

Specification

Headstage weight 3g
Headstage size 15 x 15 x 22 mm
Excitation wavelength LED peak 470 nm, Filter band 445~490 nm
Emission wavelength Filter band 500~550 nm
Excitation power 10~300 µW@400 µm Fiber end (Adjustable)
Sampling rate 100Hz
AD resolution 16bit
Photo sensor Photo diode
Gain 1010 V/A
Battery life 2 hours@Excitation power 30 µW
Transmission band 2.4GHz
Transmission distance 2m
Power Battery powered, rechargeable
Receiver I/O 1x Photometry analog out, 1x Geneal purpose analog In (0~5V)
PC Interface USB / TeleFipho software (for Windows10)
Cannula core: 400 µm / NA0.39, Cladding: 425 µm, Ferrule: 2.5 mm
core: 200 µm / NA0.39, Cladding: 225 µm, Ferrule: 2.5 mm
core: 600 µm / NA0.39, Cladding: 630 µm, Ferrule: 2.5 mm

 

TeleFipho Standard Set

TeleFipho Standard Set consists of the following items:

  • TeleFiT 1x TeleFipho Transmitter Headstage
  • TeleFiR 1x TeleFipho Receiver
  • TeleFiCharger 1x TeleFipho Charger
  • TeleFiC-l-d (※) 3x TeleFipho Cannula
  • TeleFiTool 1x Insertion Tool for TeleFipho
  • TeleFiDummy 1x TeleFipho Dummy Headstage
  • 1x TeleFipho software installer
  • 1x pMAT Converter software installer

(※) Please specify cannula length and fiber diameters. 5 mm length & 400 µm core cannula will come without specifying

TeleFiC-l-d l: length in mm (resolution 0.1 mm) / d: core diameter in µm (400 or 200)

e.g. Cannula length 3.5mm & core 400µm: TeleFiC-3.5-400

Transmitter Headstage Receiver
Charger Cannula
Insertion Tool Dummy Headstage

 

Stand alone / External recorder

TeleFipho receiver can be directly connected to PC via USB for fiber photometry data recording in TeleFipho software. TeleFipho software is good enough for simple raw data / low pass filtered data recording

Data can be directly read from Matlab / Octave, or can be exported to ASCII format

We recommend the open-source pMAT software for delta F/F and PETH and analysis, developed by Dr. David Barker in Rutgers University. For more details please see the following support page:

Analyze TeleFipho Data in pMAT software

For more complicated online analysis and / or parallel recording of other physiological data, you can access photometry data as analog signal via BNC on the front panel of TeleFipho receiver so that you can connect external data recording system (e.g. PowerLab from ADI)

Model Description
TeleFipho-set TeleFipho Standard Set
TeleFiT TeleFipho Transmitter Headstage
TeleFiR TeleFipho Receiver
TeleFiCharger TeleFipho Charger
TeleFiC-l-d TeleFipho Cannula
TeleFiTool Insertion Tool for TeleFipho
TeleFiDummy TeleFipho Dummy Headstage